High-resolution bioguided screening of Ganoderma capense for P-GP modulating activity

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dc.contributor.author Ta, Hp
dc.contributor.author Maciuk, A.
dc.contributor.author Vautier, S.
dc.contributor.author Milane, A.
dc.contributor.author Welti, S.
dc.contributor.author Champy, P.
dc.contributor.author Wainer, I.W.
dc.contributor.author Fernandez, C.
dc.contributor.author Figadere, B.
dc.date.accessioned 2019-10-15T08:18:44Z
dc.date.available 2019-10-15T08:18:44Z
dc.date.copyright 2008 en_US
dc.date.issued 2019-10-15
dc.identifier.issn 1439-0221 en_US
dc.identifier.uri http://hdl.handle.net/10725/11447
dc.description.abstract Ganoderma lucidum (Polyporaceae) is commonly used as a panacea e.g. in Traditional Chinese Medicine. Abundant research has shown its activity on several targets related to cancer, but no work assessed its modulating activity on P-gp transporter. We extracted metabolites from a clearly defined cultivated strain using petroleum ether, dichloromethane, acetonitrile, methanol and water. Water fraction was added ethanol to precipitate polysaccharides and separate them from supernatant. The 6 resulting fractions have been tested using three different cellular uptake-efflux tests (calceine, rhodamine and 3H- digoxine) in a 96-well format, using rat brain endothelium cells expressing P-gp exclusively. A systematic approach using multiple controls allowed us to record unequivocal data and gave evidence for the special procedure requirements for testing P-gp modulating activity of natural extracts. Triterpenes from the active dichloromethane fraction were isolated using a semi-preparative column and LC-MS monitoring. Compounds were tested at normalized doses, based on peaks areas detected by ELSD detection. Data are presented along with a literature review on P-gp modulating activity of plant triterpenes. Results give a rationale for the use of Ganoderma lucidum in the treatment of cancer and indicate potential pharmacokinetic interaction or potentiation of Ganoderma lucidum with other drug treatments. en_US
dc.language.iso en en_US
dc.title High-resolution bioguided screening of Ganoderma capense for P-GP modulating activity en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200904164 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Planta Medica en_US
dc.journal.volume 74 en_US
dc.journal.issue 9 en_US
dc.article.pages PC85 en_US
dc.identifier.ctation Ta, H. P., Maciuk, A., Vautier, S., Milane, A., Welti, S., Champy, P., ... & Figadère, B. (2008). High-resolution bioguided screening of Ganoderma capense for P-GP modulating activity. Planta Medica, 74(09), PC85. en_US
dc.author.email aline.milane@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0028-1084603 en_US
dc.orcid.id https://orcid.org/0000-0002-3159-6578 en_US
dc.author.affiliation Lebanese American University en_US

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