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| dc.contributor.author | Xian, Lingling | |
| dc.contributor.author | Chia, Lionel | |
| dc.contributor.author | Georgess, Dan | |
| dc.contributor.author | Shuai, Shuai | |
| dc.contributor.author | Ewald, Andrew J. | |
| dc.contributor.author | Resar, Linda M.S. | |
| dc.date.accessioned | 2019-09-20T12:14:29Z | |
| dc.date.available | 2019-09-20T12:14:29Z | |
| dc.date.copyright | 2019 | en_US |
| dc.date.issued | 2019-09-20 | |
| dc.identifier.issn | 1940-087X | en_US |
| dc.identifier.uri | http://hdl.handle.net/10725/11323 | |
| dc.description.abstract | We describe step-by-step instructions to: 1) efficiently engineer intestinal organoids using magnetic nanoparticles for lenti- or retroviral transduction, and, 2) generate frozen sections from engineered organoids. This approach provides a powerful tool to efficiently alter gene expression in organoids for investigation of downstream effects. | en_US |
| dc.language.iso | en | en_US |
| dc.title | Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning | en_US |
| dc.type | Article | en_US |
| dc.description.version | Published | en_US |
| dc.author.school | SAS | en_US |
| dc.author.idnumber | 201900498 | en_US |
| dc.author.department | Natural Sciences | en_US |
| dc.description.embargo | N/A | en_US |
| dc.relation.journal | Journal of Visualized Experiments | en_US |
| dc.journal.volume | 147 | en_US |
| dc.article.pages | 1-9 | en_US |
| dc.identifier.doi | https://doi.org/10.3791/57040 | en_US |
| dc.identifier.ctation | Xian, L., Chia, L., Georgess, D., Luo, L., Shuai, S., Ewald, A. J., & Resar, L. M. (2019). Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning. JoVE (Journal of Visualized Experiments), (147), e57040. | en_US |
| dc.author.email | dan.georgess@lau.edu.lb | en_US |
| dc.identifier.tou | http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php | en_US |
| dc.identifier.url | https://www.jove.com/video/57040/genetic-engineering-primary-mouse-intestinal-organoids-using-magnetic | en_US |
| dc.orcid.id | https://orcid.org/0000-0003-3075-4527 | en_US |
| dc.author.affiliation | Lebanese American University | en_US |
