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Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning

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dc.contributor.author Xian, Lingling
dc.contributor.author Chia, Lionel
dc.contributor.author Georgess, Dan
dc.contributor.author Shuai, Shuai
dc.contributor.author Ewald, Andrew J.
dc.contributor.author Resar, Linda M.S.
dc.date.accessioned 2019-09-20T12:14:29Z
dc.date.available 2019-09-20T12:14:29Z
dc.date.copyright 2019 en_US
dc.identifier.issn 1940-087X en_US
dc.identifier.uri http://hdl.handle.net/10725/11323
dc.description.abstract We describe step-by-step instructions to: 1) efficiently engineer intestinal organoids using magnetic nanoparticles for lenti- or retroviral transduction, and, 2) generate frozen sections from engineered organoids. This approach provides a powerful tool to efficiently alter gene expression in organoids for investigation of downstream effects. en_US
dc.language.iso en en_US
dc.title Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201900498 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Visualized Experiments en_US
dc.journal.volume 147 en_US
dc.article.pages 1-9 en_US
dc.identifier.doi https://doi.org/10.3791/57040 en_US
dc.identifier.ctation Xian, L., Chia, L., Georgess, D., Luo, L., Shuai, S., Ewald, A. J., & Resar, L. M. (2019). Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning. JoVE (Journal of Visualized Experiments), (147), e57040. en_US
dc.author.email dan.georgess@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.jove.com/video/57040/genetic-engineering-primary-mouse-intestinal-organoids-using-magnetic en_US
dc.orcid.id https://orcid.org/0000-0003-3075-4527 en_US
dc.author.affiliation Lebanese American University en_US


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