Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning

Xian, Lingling; Chia, Lionel; Georgess, Dan; Shuai, Shuai; Ewald, Andrew J.; Resar, Linda M.S.

Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning

Xian, Lingling; Chia, Lionel; Georgess, Dan; Shuai, Shuai; Ewald, Andrew J.; Resar, Linda M.S.

URI: http://hdl.handle.net/10725/11323

URL: https://www.jove.com/video/57040/genetic-engineering-primary-mouse-intestinal-organoids-using-magnetic

DOI: https://doi.org/10.3791/57040

Date: 2019-09-20

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Abstract:

We describe step-by-step instructions to: 1) efficiently engineer intestinal organoids using magnetic nanoparticles for lenti- or retroviral transduction, and, 2) generate frozen sections from engineered organoids. This approach provides a powerful tool to efficiently alter gene expression in organoids for investigation of downstream effects.

Citation:

Xian, L., Chia, L., Georgess, D., Luo, L., Shuai, S., Ewald, A. J., & Resar, L. M. (2019). Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning. JoVE (Journal of Visualized Experiments), (147), e57040.