956. Imaging of radiation-inducible promotersu using a naturally secreted luciferase from the Marine copepod gaussia princeps

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dc.contributor.author Badr, Christian E.
dc.contributor.author Zalloua, Pierre
dc.contributor.author Breakefield, Xandra O.
dc.contributor.author Tannous, Bakhos A.
dc.date.accessioned 2019-07-22T11:13:36Z
dc.date.available 2019-07-22T11:13:36Z
dc.date.copyright 2006 en_US
dc.date.issued 2019-07-22
dc.identifier.issn 1525-0024 en_US
dc.identifier.uri http://hdl.handle.net/10725/11121
dc.description.abstract Ionizing Radiation (IR)-inducible promoters used as genetic switches for cancer therapy are promising tool for controlling therapeutic gene expression. Here we describe the use of a naturally secreted luciferase from the marine copepod Gaussia princeps (Gluc) for monitoring the activity and induction levels of different IR- sensitive promoters. The following promoters were tested: wild-type p53-activated fragment 1 (WAF1, 2.4 kb); early growth response factor (Egr-1, 550 bp); four tandem repeats of the transcriptional factor nuclear factor-kB (4NF-kB, 400 bp); and a combination of both Egr-1 and 4NF-kB promoters. These promoters were cloned upstream of the humanized Gluc cDNA in a plasmid containing the eGFP gene under the control of an immediate-early herpes simplex virus type-1 promoter. Vero2-2 (African green monkey kidney cells) were transfected with each of these constructs and irradiated at different doses of g-rays. Activity of these promoters and their response to radiation was monitored overtime by taking an aliquot of the cell-free conditioned medium, adding coelenterazine, the Gluc substrate, and measuring bioluminescence. At-least two- fold induction was detected with each promoter as observed by bioluminescence imaging of Gluc. This system can be used to monitor, in real-time, the activity as well as the effectiveness of radiation-inducible promoters to drive the expression of toxic genes for cancer therapy. en_US
dc.language.iso en en_US
dc.title 956. Imaging of radiation-inducible promotersu using a naturally secreted luciferase from the Marine copepod gaussia princeps en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 20030001 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Molecular Therapy en_US
dc.journal.volume 13 Supp. 1 en_US
dc.article.pages S369 en_US
dc.identifier.doi https://doi.org/10.1016/j.ymthe.2006.08.1048 en_US
dc.identifier.ctation Badr, C. E., Zalloua, P., Breakefield, X. O., & Tannous, B. A. (2006). 956. Imaging of Radiation-Inducible Promoters Using a Naturally Secreted Luciferase from the Marine Copepod Gaussia princeps. Molecular Therapy, 13, S369. en_US
dc.author.email pierre.zalloua@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(06)01310-4 en_US
dc.orcid.id https://orcid.org/0000-0002-8494-5081 en_US
dc.author.affiliation Lebanese American University en_US

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