Increase of satellite tobacco ringspot virus RNA initiated by inoculating circular RNA

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dc.contributor.author Buzayan, Jamal M.
dc.contributor.author Tol, Hans Van
dc.contributor.author Zalloua, Pierre A.
dc.contributor.author Bruening, George
dc.date.accessioned 2019-07-19T11:00:07Z
dc.date.available 2019-07-19T11:00:07Z
dc.date.copyright 1995 en_US
dc.date.issued 2019-07-19
dc.identifier.issn 0042-6822 en_US
dc.identifier.uri http://hdl.handle.net/10725/11097
dc.description.abstract A small satellite RNA of tobacco ringspot virus (sTRSV RNA) generates circular and linear molecules of unit length and repetitive sequence, linear multimers during replication. The phosphodiester junction joining the unit satellite RNA sequences in multimeric and circular RNA resisted base-catalyzed cleavage in circles but not in linear dimers. We postulate that junctions of multimeric satellite RNA form during synthesis of the polyribonucleotide chain, whereas those of circular RNA result from a ligation reaction that introduces a group blocking the junction 2′-hydroxyl. To test the relative effectiveness of linear and circular satellite RNAs in initiating replication, we inoculated onto bean (Phaseolus vulgaris cv Black Valentine) the four possible pairs of satellite RNA molecules, one member of each pair having the wild-type sTRSV RNA sequence and the other that of the replicating mutant 51AG/212CU, with each sequence provided as the unit circular or linear form. The relative amounts of wild-type and mutant satellite RNA sequence recovered from progeny virions reflected their relative abundances in the inoculum without regard to whether the sequence was supplied as a linear or a circular molecule. These results are consistent with models for the replication of the satellite RNA in which a circular form of the satellite RNA is a template for rolling circle transcription or is otherwise a replication intermediate or is readily converted to an intermediate. We also show that a circular form of a nonaccumulating satellite RNA mutant induced an increase in a satellite RNA that is endogenous to some tobacco ringspot virus virion preparations, as demonstrated previously for the linear form. en_US
dc.language.iso en en_US
dc.title Increase of satellite tobacco ringspot virus RNA initiated by inoculating circular RNA en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 20030001 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Virology en_US
dc.journal.volume 208 en_US
dc.journal.issue 2 en_US
dc.article.pages 832-837 en_US
dc.identifier.doi https://doi.org/10.1006/viro.1995.1221 en_US
dc.identifier.ctation Buzayan, J. M., Van Tol, H., Zalloua, P. A., & Bruening, G. (1995). Increase of satellite tobacco ringspot virus RNA initiated by inoculating circular RNA. Virology, 208(2), 832-837. en_US
dc.author.email pierre.zalloua@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.sciencedirect.com/science/article/pii/S0042682285712214 en_US
dc.orcid.id https://orcid.org/0000-0002-8494-5081 en_US
dc.author.affiliation Lebanese American University en_US

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