Target-specific agents imaging ectopic and orthotopic human pancreatic cancer xenografts

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dc.contributor.author Wang, Wei
dc.contributor.author Lin, Jei
dc.contributor.author Guha, Sushovan
dc.contributor.author Tong, Zhimin
dc.contributor.author Cameron, Arlin G.
dc.contributor.author Zhang, Fujun
dc.contributor.author Qui, Xiuchun
dc.contributor.author Zou, Chaoxia
dc.contributor.author Gao, Xu
dc.contributor.author Mawad, Michel E.
dc.contributor.author Ke, Shi
dc.date.accessioned 2019-07-10T08:36:22Z
dc.date.available 2019-07-10T08:36:22Z
dc.date.copyright 2011 en_US
dc.date.issued 2019-07-10
dc.identifier.issn 1536-4828 en_US
dc.identifier.uri http://hdl.handle.net/10725/11025
dc.description.abstract Objectives: This study aimed to develop target-specific binding agents for in vitro and in vivo imaging of human pancreatic cancer. Methods: A monoclonal neutrophil gelatinase-associated lipocalin (NGAL)-specific antibody and a peptide specific for matrix metalloproteinase (MMP) were labeled with a near-infrared dye for in vitro and in vivo imaging studies. Fluorescence or confocal microscopy was used to determine antibody or peptide binding and internalization of agents into human AsPC-1, Panc-1, and MiaPaCa pancreatic cancer cell lines and in mice bearing ectopic or orthotopic pancreatic tumor transplants. Results: Both the NGAL-specific antibody and MMP peptide bound to pancreatic cancer cells with high specificity; most NGAL-specific antibody localized to the cytosol. In vivo imaging results demonstrated high signal intensity of both agents bound to the tumor. The average tumortr-to-background ratio of antibody and peptide was 1.29 and 2.86, respectively. Signal was also detectable in the liver, kidneys, and bladder. Conclusions: Both NGAL-specific antibody and MMP peptide bound to cancer cells, and the labeled antibody was internalized. These results demonstrate that both agents can be used to enhance detection of human pancreatic cancer xenografts. However, the biodistribution patterns of these agents might limit their use in research and clinical practice en_US
dc.language.iso en en_US
dc.title Target-specific agents imaging ectopic and orthotopic human pancreatic cancer xenografts en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 201700518 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Pancreas en_US
dc.journal.volume 40 en_US
dc.journal.issue 5 en_US
dc.article.pages 689-694 en_US
dc.identifier.doi https://doi.org/10.1097/MPA.0b013e31821f6b14 en_US
dc.identifier.ctation Wang, W., Lin, J., Guha, S., Tong, Z., Cameron, A. G., Zhang, F., ... & Ke, S. (2011). Target-specific agents imaging ectopic and orthotopic human pancreatic cancer xenografts. Pancreas, 40(5), 689-694. en_US
dc.author.email michel.mawad@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://oce.ovid.com/article/00006676-201107000-00010/HTML en_US
dc.author.affiliation Lebanese American University en_US

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