Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer

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dc.contributor.author Adams, Kristen E.
dc.contributor.author Ke, Shi
dc.contributor.author Kwon, Sunkuk
dc.contributor.author Liang, Feng
dc.contributor.author Fan, Zhen
dc.contributor.author Lu, Yang
dc.contributor.author Hirshi, Karen
dc.contributor.author Mawad, Michel E.
dc.contributor.author Barry, Micheal
dc.contributor.author Sevick-Muraca, Eva Marie
dc.date.accessioned 2019-07-08T06:55:51Z
dc.date.available 2019-07-08T06:55:51Z
dc.date.copyright 2007 en_US
dc.date.issued 2019-07-08
dc.identifier.issn 1560-2281 en_US
dc.identifier.uri http://hdl.handle.net/10725/10989
dc.description.abstract Targeted fluorescent molecular imaging probes may provide an optimal means of detecting disease. Stable, organic fluorophores can be repeatedly excited in vivo by propagated light and consequentially can provide large signal-to-noise ratios (SNRs) for image detection of target tissues. In the literature, many small animal imaging studies are performed with a red excitable dye, Cy5.5, conjugated to the targeting component. We report the comparison of the in vivo fluorescent imaging performance of a near-IR (NIR) and a red-excitable dye. Epidermal growth factor (EGF) was conjugated with Cy5.5 [excitation/emission (ex/em), 660/710 nm] or IRDye® 800CW (ex/em: 785/830 nm) for imaging EGF receptor (EGFr) positive (MDA-MB-468) and/or negative (MDA-MB-435) human breast cancer cell lines in subcutaneous xenograft models. The conjugates were injected intravenously at 1-nmol-dye equivalent with and without anti-EGFr monoclonal antibody C225, preadministered 24 h prior as a competitive ligand to EGFr. Our images show that while both agents target EGFr, the EGF-IRDye® 800CW evidenced a significantly reduced background and enhanced the tumor-to-background ratio (TBR) compared to the EGF-Cy5.5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered. en_US
dc.language.iso en en_US
dc.title Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 201700518 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Biomedical Optics en_US
dc.journal.volume 12 en_US
dc.journal.issue 2 en_US
dc.article.pages 024017 en_US
dc.keywords Tumors en_US
dc.keywords Tissues en_US
dc.keywords In vivo imaging en_US
dc.keywords Luminescence en_US
dc.keywords Near infrared en_US
dc.keywords Receptors en_US
dc.keywords Cancer en_US
dc.identifier.doi https://doi.org/10.1117/1.2717137 en_US
dc.identifier.ctation Adams, K. E., Ke, S., Kwon, S., Liang, F., Fan, Z., Lu, Y., ... & Sevick-Muraca, E. M. (2007). Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer. Journal of biomedical optics, 12(2), 024017. en_US
dc.author.email michel.mawad@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-12/issue-2/024017/Comparison-of-visible-and-near-infrared-wavelength-excitable-fluorescent-dyes/10.1117/1.2717137.full en_US
dc.author.affiliation Lebanese American University en_US

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