MicroRNA profiling in intraocular medulloepitheliomas

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dc.contributor.author Edward, Deepak P.
dc.contributor.author Alkatan, Hind
dc.contributor.author Qundeel, Rafiq
dc.contributor.author Eberhart, Charles
dc.contributor.author Al Mesfer, Saleh
dc.contributor.author Ghazi, Nicola
dc.contributor.author Al Safieh, Leen
dc.date.accessioned 2019-06-14T11:05:05Z
dc.date.available 2019-06-14T11:05:05Z
dc.date.copyright 2015 en_US
dc.date.issued 2019-06-14
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/10725/10828
dc.description.abstract Purpose To study the differential expression of microRNA (miRNA) profiles between intraocular medulloepithelioma (ME) and normal control tissue (CT). Material and Methods Total RNA was extracted from formalin fixed paraffin embedded (FFPE) intraocular ME (n=7) and from age matched ciliary body controls (n=8). The clinical history and phenotype was recorded. MiRNA profiles were determined using the Affymetrix GeneChip miRNA Arrays analyzed using expression console 1.3 software. Validation of significantly dysregulated miRNA was confimed by quantitaive real-time PCR. The web-based DNA Intelligent Analysis (DIANA)-miRPath v2.0 was used to perform enrichment analysis of differentially expressed (DE) miRNA gene targets in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Results The pathologic evaluation revealed one benign (benign non-teratoid, n=1) and six malignant tumors (malignant teratoid, n=2; malignant non-teratoid, n = 4). A total of 88 miRNAs were upregulated and 43 miRNAs were downregulated significantly (P<0.05) in the tumor specimens. Many of these significantly dysregulated miRNAs were known to play various roles in carcinogenesis and tumor behavior. RT-PCR validated three significantly upregulated miRNAs and three significantly downregulated miRNAs namely miR-217, miR-216a, miR-216b, miR-146a, miR-509-3p and miR-211. Many DE miRNAs that were significant in ME tumors showed dysregulation in retinoblastoma, glioblastoma, and precursor, normal and reactive human cartilage. Enriched pathway analysis suggested a significant association of upregulated miRNAs with 15 pathways involved in prion disease and several types of cancer. The pathways involving significantly downregulated miRNAs included the toll-like receptor (TLR) (p<4.36E-16) and Nuclear Factor kappa B (NF-κB) signaling pathways (p<9.00E-06). Conclusions We report significantly dysregulated miRNAs in intraocular ME tumors, which exhibited abnormal profiles in other cancers as well such as retinoblastoma and glioblastoma. Pathway analysis of all dysregulated miRNAs shared commonalities with other cancer pathways. en_US
dc.language.iso en en_US
dc.title MicroRNA profiling in intraocular medulloepitheliomas en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 201000154 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Plos one en_US
dc.journal.volume 10 en_US
dc.journal.issue 3 en_US
dc.article.pages e0121706 en_US
dc.identifier.doi https://doi.org/10.1371/journal.pone.0121706 en_US
dc.identifier.ctation Edward, D. P., Alkatan, H., Rafiq, Q., Eberhart, C., Al Mesfer, S., Ghazi, N., ... & Amero, K. K. A. (2015). MicroRNA profiling in intraocular medulloepitheliomas. PloS one, 10(3), e0121706. en_US
dc.author.email nicola.ghazi@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121706 en_US
dc.author.affiliation Lebanese American University en_US

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