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Chemical composition, antioxidant and anti-inflammatory activity evaluation of the lebanese propolis extract

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dc.contributor.author Rawan, Zeitoun
dc.contributor.author Najjar, Fadia
dc.contributor.author Wehbi, Batoul
dc.contributor.author Khalil, Alia
dc.contributor.author Fayyad-Kazan, Mohammad
dc.contributor.author Dagher-Hamalian, Carole
dc.contributor.author Faour, Wissam H.
dc.contributor.author El-Makhour, Yolla
dc.date.accessioned 2019-05-23T12:17:10Z
dc.date.available 2019-05-23T12:17:10Z
dc.date.copyright 2019 en_US
dc.identifier.issn 1873-4316 en_US
dc.identifier.uri http://hdl.handle.net/10725/10663
dc.description.abstract Background: Propolis is a resinous substance produced by bees and known to possess antioxidant, antimicrobial, antiproliferative and anti-inflammatory activities. Objective: This study is aimed at evaluating the in vivo and in vitro anti-inflammatory potential of the Crude Ethanolic Extract (CE) of Lebanese propolis and its Ethyl Acetate Fraction (EAF). Method: Chemical content of propolis was characterized using high-performance liquid chromatography and LC-MS/MS. COX-2 and iNOS protein expression, nitric oxide (NO) and prostaglandin (PGE2) release in LPS-activated RAW monocytes were achieved respectively by western blot and spectrophotometry. Antioxidant activity was evaluated by DPPH free radical scavenging assay. Measurement of paw thickness in carrageenan-induced paw edema in mice and pathologic assessment of inflammation in paw sections were used to judge the anti-inflammatory properties of propolis. Results: Pathology analysis revealed in the treated group significant reduction of immune cell infiltration and edema. Both extract and ethyl acetate fraction showed significant anti-inflammatory and antioxidant effects in LPS-treated RAW cells characterized by the inhibition of COX-2 and iNOS protein expression, as well as PGE2 and NO release. Chemical analysis of the crude extract and its ethyl acetate fraction identified 28 different compounds of which two phenolic acids and nine other flavonoids were also quantified. Ferulic acid, caffeic acid, chrysin, galangin, quercetin, and pinocembrin were among the most representative compounds. Conclusion: Lebanese propolis is rich in a various amount of flavonoids which showed promising antiinflammatory and antioxidant properties. Additionally, chemical analysis showed unique chemical compositions with the potential of identifying ingredients with interesting anti-inflammatory activities. en_US
dc.language.iso en en_US
dc.title Chemical composition, antioxidant and anti-inflammatory activity evaluation of the lebanese propolis extract en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 200902770 en_US
dc.author.idnumber 200904962 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Current Pharmaceutical Biotechnology en_US
dc.journal.volume 20 en_US
dc.journal.issue 1 en_US
dc.article.pages 84-96 en_US
dc.keywords Lebanese propolis en_US
dc.keywords Antioxidant en_US
dc.keywords Chromatography en_US
dc.keywords Flavonoids en_US
dc.keywords Inflammation en_US
dc.keywords Phenolic acids en_US
dc.identifier.doi https://doi.org/10.2174/1389201020666190206201241 en_US
dc.identifier.ctation Zeitoun, R., Najjar, F., Wehbi, B., Khalil, A., Fayyad-Kazan, M., Dagher-Hamalian, C., ... & El-Makhour, Y. (2019). Chemical Composition, Antioxidant and Anti-inflammatory Activity Evaluation of the Lebanese Propolis Extract. Current pharmaceutical biotechnology, 20(1), 84-96. en_US
dc.author.email carole.hamalian@lau.edu.lb en_US
dc.author.email wissam.faour@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.ingentaconnect.com/contentone/ben/cpb/2019/00000020/00000001/art00011 en_US
dc.author.affiliation Lebanese American University en_US


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