Proteome analysis of Candida albicans cell wall mutants. (c2011)

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dc.contributor.author Koussa, Joseph Wissam
dc.date.accessioned 2012-01-18T12:05:56Z
dc.date.available 2012-01-18T12:05:56Z
dc.date.copyright 2011 en_US
dc.date.issued 2012-01-18
dc.date.submitted 2011-08-23
dc.identifier.uri http://hdl.handle.net/10725/1050
dc.description Includes bibliographical references (leaves 51-56). en_US
dc.description.abstract The fungal pathogen Candida albicans is a leading causative agent of death in immuno-compromised individuals. This pathogenicity involves, among other, morphological switching from yeast to hyphal form. When filamentation is induced, major structural and compositional alterations take place in the cell wall affecting cell wall chitin content and cell wall proteome composition. Knockouts of pir32, hwp2and pga1, three cell wall protein coding genes, have been previously generated by homologous recombination of marker cassettes in our lab. The purpose of this current study is to analyze the role these genes play by comparing the cell wall composition of the mutant strains to the parental wild type strain under both filamentous and non filamentous conditions. Cell wall isolation and total cell wall protein content of these strains showed alterations in the cell wall proteome. Chitin content was found to significantly decrease(50%) in the pga1/pga1 mutant under non filamentous conditions, whereas in the pir32/pi32 mutant, and under similar conditions, a 150% increase in chitin content was recorded, an interesting result bearing in mind that chitin is a key player in cell wall structure and rigidity. Total cell wall protein content ranged from a 50% decrease in the pga1 null to a 50% increase in the pir32 mutant, implying fundamental changes in the cell wall proteome. MALDI-TOF analysis showed differential peptide mass fingerprints of cell wall proteins with many peaks found to be unique to each mutant and to specific growth conditions. These peaks, when entered into available databases, remained unidentified. As such, future work requires analysis of these peaks by MS/MS peptide sequencing in an effort to identify specific up or down regulated proteins represented by these peaks. en_US
dc.language.iso en en_US
dc.subject Candida albicans en_US
dc.subject Proteomics en_US
dc.subject Fungal cell walls en_US
dc.title Proteome analysis of Candida albicans cell wall mutants. (c2011) en_US
dc.type Thesis en_US
dc.term.submitted Summer II en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school Arts and Sciences en_US
dc.author.idnumber 200804473 en_US
dc.author.commembers Dr. Brigitte Wex
dc.author.commembers Dr. Sima Tokajian
dc.author.commembers Dr. Mirvat Al Sibai
dc.author.woa OA en_US
dc.description.physdesc 1 bound copy: xi, 73 leaves; ill.; 30 cm. available at RNL. en_US
dc.author.division Biology en_US
dc.author.advisor Dr. Roy Khalaf
dc.keywords Cell wall Proteins en_US
dc.keywords Chitin en_US
dc.keywords MALDI-TOF MS en_US
dc.keywords C. albicans en_US
dc.identifier.doi https://doi.org/10.26756/th.2011.47 en_US
dc.publisher.institution Lebanese American University en_US

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