Breast cancer cells and bone marrow mesenchymal stromal cells

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dc.contributor.author Faour, Wissam H.
dc.contributor.author Najar, Mehdi
dc.contributor.author Fayyad-Kazan, Hussein
dc.contributor.author Badran, Bassam
dc.contributor.author Journe, Fabrice
dc.contributor.author Lagneaux, Laurence
dc.date.accessioned 2019-04-25T07:01:20Z
dc.date.available 2019-04-25T07:01:20Z
dc.date.copyright 2017 en_US
dc.date.issued 2019-04-25
dc.identifier.issn 1420-908X en_US
dc.identifier.uri http://hdl.handle.net/10725/10498
dc.description.abstract Objective The role of direct cell–cell interactions mediating selective bone metastasis by breast cancer cells (BCCs) niche is still mostly unknown. Materials and methods Conditioned medium and direct cell–cell contacts experiments were used to investigate the effect of bone marrow-derived mesenchymal stromal cells (MSCs), osteoprogenitor-like cells (MG-63) and osteosarcoma cells (SaOS-2) on luminal-like (MCF-7) and basal-like (MDA-MB-231) BCCs flow cytometry was used to assess the purity of isolated BCCs and osteoblasts. Expression of osteoblastic markers was investigated by semi-quantitative RT-PCR. RANKL and OPG levels were measured by ELISA. Results Conditioned medium from MSCs and osteoblasts induced the expression of osteoblastic markers in BCCs. While co-culture assays with SaOS-2 increased the expression of osteoblastic markers in MCF-7 cells, SaOS-2 cell conditioned medium increased the expression of RANKL, PTHrP, VEGF and NOGGIN in MCF-7 cells. Co-cultures with either MG-63 cells or MSCs induced OPG and MMP-2 in both tumor cell lines. Interestingly, conditioned medium from co-cultures of MSCs and MDA-MB-231 cells significantly decreased the proliferation of activated T lymphocytes which was reversed by addition of anti-OPG antibodies to the co-cultures. Conclusion Our data suggest that MSCs strongly contribute to the adaptation and invasiveness of breast cancer cells in skeletal tissues. en_US
dc.language.iso en en_US
dc.title Breast cancer cells and bone marrow mesenchymal stromal cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.title.subtitle a regulated modulation of the breast tumor in the context of immune response en_US
dc.author.school SOM en_US
dc.author.idnumber 200904962 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Inflammation Research en_US
dc.journal.volume 66 en_US
dc.journal.issue 2 en_US
dc.article.pages 129-139 en_US
dc.keywords Breast cancer cells en_US
dc.keywords Bone metastasis en_US
dc.keywords Osteoprogenitor cells en_US
dc.keywords Mesenchymal stromal cells en_US
dc.identifier.ctation Najar, M., Fayyad-Kazan, H., Faour, W. H., Badran, B., Journe, F., & Lagneaux, L. (2017). Breast cancer cells and bone marrow mesenchymal stromal cells: a regulated modulation of the breast tumor in the context of immune response. Inflammation Research, 66(2), 129-139. en_US
dc.author.email wissam.faour@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://link.springer.com/article/10.1007/s00011-016-1000-8 en_US
dc.author.affiliation Lebanese American University en_US

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