Immuno-biological comparison of hepatic stellate cells in a reverted and activated state

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dc.contributor.author Faour, Wissam H.
dc.contributor.author Najar, Mehdi
dc.contributor.author Fayyad-Kazan, Hussein
dc.contributor.author El Taghdouini, Adil
dc.contributor.author Raicvic, Gordana
dc.contributor.author van Grunsven, Leo A.
dc.contributor.author Najimi, Mustapha
dc.contributor.author Sokal, Etienne
dc.contributor.author Lagneaux, Laurence
dc.date.accessioned 2019-04-23T13:28:33Z
dc.date.available 2019-04-23T13:28:33Z
dc.date.copyright 2018 en_US
dc.date.issued 2019-04-23
dc.identifier.issn 1950-6007 en_US
dc.identifier.uri http://hdl.handle.net/10725/10494
dc.description.abstract Human hepatic stellate cells (HSCs) demonstrated great immunological plasticity with important consequences for liver cell therapy. Activated HSCs (aHSCs) are in vitro reverted (rHSCs) to a quiescent-like phenotype with potential benefit to reduce liver fibrosis. The goal of this study is to establish and compare the immunological profile of activated and in vitro reverted HSCs and to investigate the impact of inflammatory priming on the immunobiology of both HSCs populations. The distribution of inflammatory primed activated and reverted HSCs across the different phases of the cell cycle is assessed by flow cytometry. In addition, Flow analysis was done to assess the expression level of neuronal, endothelial and stromal markers, cell adhesion molecules, human leucocyte antigens, co-stimulatory molecules, immunoregulatory molecules and natural killer ligands. Our results showed that the cell cycle distribution of both HSCs populations is significantly modulated by inflammation. Accordingly, activated HSC that were in G1 phase switch to S- and G2 phases when exposed to inflammation, while reverted HSCs mostly redistribute into sub-G0 phase. In a HSC state dependent manner, inflammatory priming modulated the expression of the stromal marker CD90, biological receptors (CD95 and CD200R), cell adhesion molecules (CD29, CD54, CD58, CD106 and CD166), human leucocyte antigen HLA-G, co-stimulatory molecules (CD40 and CD252), as well as the immunoregulatory molecules (CD200 and CD274). In conclusion, the immunologic profile of HSCs is significantly modulated by their activation state and inflammation and is important for the development of novel HSC liver cell-based therapy. en_US
dc.language.iso en en_US
dc.title Immuno-biological comparison of hepatic stellate cells in a reverted and activated state en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Biomedicine & Pharmacotherapy en_US
dc.journal.volume 98 en_US
dc.article.pages 52-62 en_US
dc.keywords Hepatic stellate cell en_US
dc.keywords Activation en_US
dc.keywords Reversion en_US
dc.keywords Inflammation en_US
dc.keywords Cell cycle en_US
dc.identifier.doi https://doi.org/10.1016/j.biopha.2017.12.027 en_US
dc.identifier.ctation Najar, M., Fayyad-Kazan, H., Faour, W. H., El Taghdouini, A., Raicevic, G., van Grunsven, L. A., ... & Lagneaux, L. (2018). Immuno-biological comparison of hepatic stellate cells in a reverted and activated state. Biomedicine & Pharmacotherapy, 98, 52-62. en_US
dc.author.email wissam.faour@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.sciencedirect.com/science/article/pii/S0753332217351302 en_US
dc.author.affiliation Lebanese American University en_US

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