Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination

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dc.contributor.author Georgess, Dan
dc.contributor.author Shamir, Eliah R.
dc.contributor.author Coutinho, Kester
dc.contributor.author Auer, Manfred
dc.contributor.author Elwald, Andrew J.
dc.date.accessioned 2019-04-04T11:46:55Z
dc.date.available 2019-04-04T11:46:55Z
dc.date.copyright 2016 en_US
dc.date.issued 2019-04-04
dc.identifier.issn 2046-6390 en_US
dc.identifier.uri http://hdl.handle.net/10725/10342
dc.description.abstract Dissemination is the process by which cells detach and migrate away from a multicellular tissue. The epithelial-to-mesenchymal transition (EMT) conceptualizes dissemination in a stepwise fashion, with downregulation of E-cadherin leading to loss of intercellular junctions, induction of motility, and then escape from the epithelium. This gain of migratory activity is proposed to be mutually exclusive with proliferation. We previously developed a dissemination assay based on inducible expression of the transcription factor Twist1 and here utilize it to characterize the timing and dynamics of intercellular adhesion, proliferation and migration during dissemination. Surprisingly, Twist1+ epithelium displayed extensive intercellular junctions, and Twist1– luminal epithelial cells could still adhere to disseminating Twist1+ cells. Although proteolysis and proliferation were both observed throughout dissemination, neither was absolutely required. Finally, Twist1+ cells exhibited a hybrid migration mode; their morphology and nuclear deformation were characteristic of amoeboid cells, whereas their dynamic protrusive activity, pericellular proteolysis and migration speeds were more typical of mesenchymal cells. Our data reveal that epithelial cells can disseminate while retaining competence to adhere and proliferate. en_US
dc.language.iso en en_US
dc.title Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201900498 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Biology Open en_US
dc.journal.volume 2016 en_US
dc.journal.issue 5 en_US
dc.article.pages 1216-1228 en_US
dc.keywords Twist1 en_US
dc.keywords Dissemination en_US
dc.keywords Epithelial-mesenchymal transition en_US
dc.keywords Cell migration en_US
dc.keywords Intercellular junctions en_US
dc.keywords Proteolysis en_US
dc.identifier.ctation Shamir, E. R., Coutinho, K., Georgess, D., Auer, M., & Ewald, A. J. (2016). Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination. Biology open, 5(9), 1216-1228. en_US
dc.author.email dan.georgess@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://bio.biologists.org/content/5/9/1216?utm_source=Sub&utm_medium=BiO_JCS&utm_campaign=BiO_Snippet&utm_content=5-1216 en_US
dc.author.affiliation Lebanese American University en_US

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