Kefir inhibits proliferation and induces apoptosis of epithelial colorectal adenocarcinoma cells. (c2011)

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dc.contributor.author Hayek, Stephanie E.
dc.date.accessioned 2011-11-18T13:20:14Z
dc.date.available 2011-11-18T13:20:14Z
dc.date.copyright 2011 en_US
dc.date.issued 2011-11-18
dc.date.submitted 2011-08-03
dc.identifier.uri http://hdl.handle.net/10725/1016
dc.description Includes bibliographical references (leaves 50-57). en_US
dc.description.abstract Kefir is a fermented milk product, to which many health benefits have been attributed. These include anti-microbial, anti-inflammatory, immunomodulatory, and metabolic benefits. Most importantly, many studies have proven that kefir may have anti-cancerous potential. The current study aims at examining the effect of this probiotic on colorectal cancer cells, in vitro. Kefir’s anti-cancer potential was tested on Caco-2 and HT-29, colorectal adenocarcinoma cell lines, through cytotoxicity, proliferation, and apoptotic assays. Kefir’s cytotoxicity was assessed by determining the percentage viability of cells, using the Trypan Blue Exclusion method. After determining the IC50, the effect of various concentrations of kefir on the proliferation of Caco-2 and HT-29 cells was determined. Using WST-1 cell proliferation assay, kefir was shown to be anti-proliferative to the cells in a dose and time dependent manner. Through flow cytometry, kefir was also shown to induce cell cycle arrest at the G1 phase, which was assumed to be an apoptotic effect. This was then confirmed by Cell Death Elisa Plus kit, which showed that apoptosis increased with increasing kefir concentration. Western Blot analysis demonstrated that kefir induces the overexpression of Bax, while repressing Bcl-2. en_US
dc.language.iso en en_US
dc.subject Kefir -- Health aspects en_US
dc.subject Colon (Anatomy) -- Cancer -- Treatment en_US
dc.subject Apoptosis en_US
dc.subject Probiotics en_US
dc.title Kefir inhibits proliferation and induces apoptosis of epithelial colorectal adenocarcinoma cells. (c2011) en_US
dc.type Thesis en_US
dc.term.submitted Summer I en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school Arts and Sciences en_US
dc.author.idnumber 200900697 en_US
dc.author.commembers Dr. Mirvat EI-Sibai
dc.author.commembers Dr. Roula Abdd Massih
dc.author.woa OA en_US
dc.description.physdesc 1 bound copy: xiv, 57 leaves; ILL.; 30 cm. available at RNL. en_US
dc.author.division Biology en_US
dc.author.advisor Dr. Sandra Rizk-Jarnati
dc.keywords Kefir en_US
dc.keywords Colorectal Cancer en_US
dc.keywords Apoptosis en_US
dc.keywords Bax en_US
dc.keywords Bcl-2 en_US
dc.identifier.doi https://doi.org/10.26756/th.2011.27 en_US
dc.publisher.institution Lebanese American University en_US

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