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Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea

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dc.contributor.author Ansari, Mohd Ikram
dc.contributor.author Harb, Moustapha
dc.contributor.author Jones, Burton
dc.contributor.author Hong, Pei-Yung
dc.date.accessioned 2019-03-08T14:36:08Z
dc.date.available 2019-03-08T14:36:08Z
dc.date.copyright 2015 en_US
dc.identifier.issn 2045-2322 en_US
dc.identifier.uri http://hdl.handle.net/10725/10153
dc.description.abstract Molecular-based approaches were used to characterize the coastal microbiota and to elucidate the trophic state of Red Sea. Nutrient content and enterococci numbers were monitored, and used to correlate with the abundance of microbial markers. Microbial source tracking revealed the presence of >1 human-associated Bacteroides spp. at some of the near-shore sampling sites and at a heavily frequented beach. Water samples collected from the beaches had occasional exceedances in enterococci numbers, higher total organic carbon (TOC, 1.48–2.18 mg/L) and nitrogen (TN, 0.15–0.27 mg/L) than that detected in the near-shore waters. Enterococci abundances obtained from next-generation sequencing did not correlate well with the cultured enterococci numbers. The abundance of certain genera, for example Arcobacter, Pseudomonas and unclassified Campylobacterales, was observed to exhibit slight correlation with TOC and TN. Low abundance of functional genes accounting for up to 41 copies/L of each Pseudomonas aeruginosa and Campylobacter coli were detected. Arcobacter butzleri was also detected in abundance ranging from 111 to 238 copies/L. Operational taxonomic units (OTUs) associated with cyanobacteria, Prochlorococcus, Ostreococcus spp. and Gramella were more prevalent in waters that were likely impacted by urban runoffs and recreational activities. These OTUs could potentially serve as quantifiable markers indicative of the water quality. en_US
dc.language.iso en en_US
dc.title Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOE en_US
dc.author.idnumber 201806912 en_US
dc.author.department Civil Engineering en_US
dc.description.embargo N/A en_US
dc.relation.journal Scientific Reports en_US
dc.journal.volume 5 en_US
dc.journal.issue 9001 en_US
dc.identifier.doi https://doi.org/10.1038/srep09001 en_US
dc.identifier.ctation Ansari, M. I., Harb, M., Jones, B., & Hong, P. Y. (2015). Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea. Scientific reports, 5, 9001. en_US
dc.author.email moustapha.harb@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.nature.com/articles/srep09001 en_US
dc.orcid.id https://orcid.org/0000-0002-1566-987X en_US
dc.author.affiliation Lebanese American University en_US


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