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Browsing by Author "Ewald, Andrew J."

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Genetic engineering of primary mouse intestinal organoids using magnetic nanoparticle transduction viral vectors for frozen sectioning

Xian, Lingling; Chia, Lionel; Georgess, Dan; Shuai, Shuai; Ewald, Andrew J.; Resar, Linda M.S. (2019-09-20)

We describe step-by-step instructions to: 1) efficiently engineer intestinal organoids using magnetic nanoparticles for lenti- or retroviral transduction, and, 2) generate frozen sections from engineered organoids. This ...
Mosaic loss of non-muscle myosin IIA and IIB is sufficient to induce mammary epithelial proliferation

Georgess, Dan; Nguyen-Ngoc, Kim-vy; Silvestri, Vanesa; Fairchild, Armanda N.; Ewald, Andrew J. (2019-04-04)

The mammary epithelium elaborates through hormonally regulated changes in proliferation, migration and differentiation. Non-muscle myosin II (NMII) functions at the interface between contractility, adhesion and signal ...

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