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Browsing by Author "Backer, Jonathan M."

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Browsing by Author "Backer, Jonathan M."

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  • Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells 
    El-Sibai, Mirvat; Nalbant, Peri; Pang, Huan; Flinn, Rory J.; Sarmiento, Corina; Macaluso, Frank; Cammer, Michael; Condeelis, John S.; Hahn, Klaus M.; Backer, Jonathan M. (2015-11-09)
    Cdc42 plays a central role in regulating the actin cytoskeleton and maintaining cell polarity. Here, we show that Cdc42 is crucial for epidermal growth factor (EGF)- stimulated protrusion in MTLn3 carcinoma cells. ...
  • Cofilin determines the migration behavior and turning frequency of metastatic cancer cells 
    Sidani, Mazen; Wessels, Deborah; Mouneimne, Ghassan; Ghosh, Mousumi; Goswami, Sumanta; Sarmiento, Corina; Wang, Weigang; Kuhl, Spencer; El-Sibai, Mirvat; Backer, Jonathan M.; Eddy, Robert; Soll, David; Condeelis, John (2015-11-09)
    We have investigated the effects of inhibiting the expression of cofilin to understand its role in protrusion dynamics in metastatic tumor cells, in particular. We show that the suppression of cofilin expression in MTLn3 ...
  • The distinct roles of Ras and Rac in PI 3-kinase-dependent protrusion during EGF-stimulated cell migration 
    Yip, Shu-Chin; El-Sibai, Mirvat; Coniglio, Salvatore J.; Mouneimne, Ghassan; Eddy, Robert J.; Drees, Beth E.; Neilsen, Paul O.; Goswami, Sumanta; Symons, Marc; Condeelis, John S.; Backer, Jonathan M. (2015-11-09)
    Cell migration involves the localized extension of actinrich protrusions, a process that requires Class I phosphoinositide 3-kinases (PI 3-kinases). Both Rac and Ras have been shown to regulate actin polymerization ...
  • Over‐expression of the p110β but not p110α isoform of PI 3‐kinase inhibits motility in breast cancer cells 
    Yip, Shu-Chin; El-Sibai, Mirvat; Hill, Karen M.; Wu, Haiyan; Fu, Zheng; Condeelis, John S.; Backer, Jonathan M. (2015-11-09)
    Phosphoinositide 3-kinase (PI 3-kinase) activity is required for growth factor-induced cytoskeletal regulation and cell migration. We previously found that in MTLn3 rat adenocarcinoma cells, EGF-stimulated induction of ...
  • Phospholipase C negatively regulates Rac/Cdc42 activation in antigen-stimulated mast cells 
    El-Sibai, Mirvat; Backer, Jonathan M. (2015-11-09)
    The Rho GTPases Rac and Cdc42 play a central role in the regulation of secretory and cytoskeletal responses in antigen-stimulated mast cells. In this study, we examine the kinetics and mechanism of Rac and Cdc42 activation ...
  • Regulation of Class IA PI 3-kinases 
    Wu, Haiyan; Shekar, S. Chandra; Flinn, Rory J.; El-Sibai, Mirvat; Jaiswal, Bijay S.; Sen, K. Ilker; Janakiraman, Vasantharajan; Seshagiri, Somasekar; Gerfen, Gary J.; Girvin, Mark E.; Backer, Jonathan M. (2015-11-04)
    We previously proposed a model of Class IA PI3K regulation in which p85 inhibition of p110α requires (i) an inhibitory contact between the p85 nSH2 domain and the p110α helical domain, and (ii) a contact between the p85 ...
  • RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells 
    El-Sibai, Mirvat; Pertz, Olivier; Pang, Huan; Yip, Shu-Chin; Lorenz, Mike; Symons, Marc; Condeelis, John S.; Hahn, Klaus M.; Backer, Jonathan M. (2015-11-04)
    Rho GTPases are versatile regulators of cell shape that act on the actin cytoskeleton. Studies using Rho GTPase mutants have shown that, in some cells, Rac1 and Cdc42 regulate the formation of lamellipodia and filopodia, ...
  • WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells 
    Sarmiento, Corina; Wang, Weigang; Dovas, Athanassios; Yamaguchi, Hideki; Sidani, Mazen; El-Sibai, Mirvat; Desmarais, Vera; Holman, Holly A.; Kitchen, Susan; Backer, Jonathan M.; Alberts, Art; Condeelis, John (2017-02-08)
    We examined the role of the actin nucleation promoters neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE2 in cell protrusion in response to epidermal growth factor (EGF), a key regulator in carcinoma cell invasion. ...